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The 2 protein bands from forty kDa to thirty kDa were absent right after 24 h incubation at 45 °C. For the rest of the bands, various concentrations of all 3 proteases retained protein bands corresponding to their first molar masses (Determine 6a,b). The presence of bands at low molecular weight in fish roe ahead of hydrolysis was unexpected. While in the report by Ghelichi et al. [eight], slight peaks of a relatively lower molecular bodyweight array (3–14 kDa) were noticed in defatted carp roe homogenate, potentially on account slot gacor hari ini of an autolytic system happening in fish roe and/or perhaps the defatting system leading to the formation of very low-molecular-excess weight peptides.
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